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Protein preservation and rumen degradability of ensiled forage, previously treated with microwave or steam heat, formic acid, or anhydrous ammonia

Abstract

dc:description.abstract

Forage may undergo extensive proteolysis during fermentation. The objectives of this study were to determine if treatment of forage with heat can reduce proteolysis during subsequent fermentation. In Experiment 1, direct-cut barley and alfalfa were either microwaved or steamed then ensiled in laboratory silos as were untreated and wilted forage. Silages of microwaved or steamed forage showed marked increase in NDIN and recovery of hot water insoluble N; however, alfalfa silages also had high pH and butyric acid. In Experiment 2, steaming was compared to formic acid and anhydrous ammonia treatments for their ability to prevent proteolysis in alfalfa silages. Steamed and ensiled alfalfa also was evaluated with addition of microbial inoculant or formic acid. Silage of steamed alfalfa had greater NDIN and recovery of precipitable N than controls, formic acid, or ammonia treated silage. There was no difference in precipitable N between formic acid and ammonia treatments. Silage of steam treatment had lower pH than wilted or direct-cut controls, and additives to steamed forage favored a more homolactic fermentation. Additives to steamed forage also increased aerobic stability of the silage. Steamed silage had less aerobic stability than direct-cut silage. Rumen degradability of silage CP and OM from both experiments were evaluated. In Experiment 1, CP degradability of microwaved or steamed silages was 8 to 26% less than unheated silages, but all had similar undegraded CP after incubation for 72 h. In Experiment 2, wilting, steam, formic acid and ammonia treatments had similar, but decreased CP degradability when compared to direct-cut silage. Longer duration heat in Experiment 1 resulted in greater silage protein preservation, and greater decrease in rumen degradability of CP than Experiment 2.

Degree

thesis:*
Name thesis:degree_name
Master of Science
Level thesis:degree_level
masters
Discipline thesis:degree_discipline
Dairy Science
Department dc:contributor.department
Dairy Science
Grantor dc:publisher
Virginia Tech
Year dc:date.issued
1991

Author and committee

dc:creator, dc:contributor.*
Author dc:creator
  • Stieve, Dale Edward M.

Rights

dc:rights
Statement dc:rights
  • In Copyright
Language dc:language.iso
en

Identifiers

dc:identifier.*
Dc Identifier Other
etd-10312009-020048
OAI identifier oai:identifier
oai:vtechworks.lib.vt.edu:10919/45338

Chain of custody

source
Harvested from
Virginia Tech
Base URL
vtechworks.lib.vt.edu/oai/request
Last updated
2026-07-22
Source record
OAI-PMH GetRecord
related terms
citation

Stieve, Dale Edward M.. Protein preservation and rumen degradability of ensiled forage, previously treated with microwave or steam heat, formic acid, or anhydrous ammonia. masters thesis, Virginia Tech, 1991. http://hdl.handle.net/10919/45338