The isolation and characterization of a growth factor in rumen fluid for a strain of Butyrivibrio

One or more factors which occur in bovine rumen fluid stimulate the growth of a strain of Butyrivibrio. The stimulating material is heat stable, organic in nature and non-dialyzable. It cannot be extracted from rumen fluid with lipid solvents and is retained in part on anion and cation exchange resins. It can be eluted from the resins with strong acid. It is stable to enzymatic hydrolysis by trypsin. Granular mucin or bovine saliva will partially replace the stimulatory activity. The part of the material which was not replaced by mucin did not appear to be any compound that is commonly used to stimulate bacterial growth. The presence of a possible inhibitor for the growth of a strain of Butyrivibrio was demonstrated.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biochemistry

Department dc:contributor.department

Biochemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1961

Author dc:creator

• Gordon, Gale Ross

Chair dc:contributor.committeechair

• Moore, Walter E. C.

Committee members dc:contributor.committeemember

• King, Kendall W.
• Kramer, Clyde Y.
• Fulghum, R. E.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-06102012-040053

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/43025