Absorption of carnosine and methionylglycine by sheep ruminal and omasal epithelia

Carnosine and methionylglycine (using ³⁵S-methionylglycine as a representative marker) absorption and transfer across ruminal and omasal epithelia collected from four (carnosine) and seven (methionylglycine) sheep were studied using parabiotic chambers that were repeatedly sampled over a 240-min incubation. The quantity of dipeptide absorbed or transferred was linearly (P < .01) dependent on substrate concentration. Carnosine was transferred intact across both tissues. More carnosine was absorbed (P < .02) and transferred (P < .01) by the omasal epithelia. Methionylglycine was transferred intact across both tissues, but less (P < .01) remained intact in serosal buffer after 240 min incubation with omasal epithelium than with ruminal epithelium. The amount of methionylglycine that accumulated in each tissue was similar. Methionylglycine accumulation in tissues plus transfer after 240 min was greater (P < .01) for omasal tissue. The ability of sheep ruminal and omasal epithelia to absorb and transfer carnosine and methionylglycine in parabiotic units was demonstrated. Dipeptide translocation across forestomach epithelial tissues, which has not been reported previously, may be an important route for supplying dietary amino acids to the ruminant.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Animal Science

Department dc:contributor.department

Animal Science

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1991

Author dc:creator

• Matthews, James C.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-03172010-020424

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/41689