Antioxidant activity of Mn-salophen complex and its effects on antioxidant enzymes in Escherichia coli

Mn-salophen complex with superoxide-scavenging activity was prepared from manganese(III) acetate dihydrate and salophen in ethanol. Visible absorption spectrum of the red-brown solution exhibited a broad absorption band at 430 - 450 nm with two shoulders between 500 and 600 nm which were absent with either salophen or manganic acetate alone. Titration of salophen with manganese(III) was consistent with a 1:1 Mn to salophen stoichiometry of the complex based on changes in the absorbance at 500 nm or of superoxide scavenging activity. The SOD-like activity of the complex in the xanthine-xanthine oxidase/cytochrome <i>c</i> assay was 1450 units/mg salophen. The SOD activity of the complex was suppressed 50% in the presence of EDTA (1 mM), but was not altered in the presence of bovine serum albumin (1 mg/ml) or crude protein extract of <i>E. coli</i> QC779 <i>sodA sodB</i> (1 mg/ml). <i>E. coli</i> QC779 <i>sodA sodB</i> grew scantily after an 8 hour lag phase in aerobic M63 glucose minimal medium.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Biochemistry and Anaerobic Microbiology

Department dc:contributor.department

Biochemistry and Anaerobic Microbiology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1994

Author dc:creator

• Liu, Zheng-Xian

Chair dc:contributor.committeechair

• Gregory, Eugene M.

Committee members dc:contributor.committeemember

• Bevan, David R.
• Bunce, George Edwin
• Hess, John L.
• Misra, Hara P.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-10202005-102838

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/40046