Action at a distance for specific repression of the glpD and glpTQ genes, and organization of the glpEGR genes of Escherichia coli K-12

Aerobic sn-glycero13-phosphate (glycerol-P) dehydrogenase is a cytoplasmic membrane-associated respiratory enzyme encoded by the g/pD gene of Escherichia coli. The divergently transcribed glpACB and glpTQ operons encode the subunits of the anaerobic glycerol-P dehydrogenase, glycerol-P transporter, and glycerophosphodiesterase, respectively. The glpD operon is controlled by cooperative binding of glp repressor (GlpR) to tandem operators that cover the -10 promoter element and 30 bp downstream of the transcription start site. The glpACB-glpTQ operons operons are controlled by GlpR binding to operators that overlap the glpA promoter elements. In this study, two additional operators were identified within the glpD structural gene, and three additional operators were identified within the glp T structural gene.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Biochemistry

Department dc:contributor.department

Biochemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1997

Author dc:creator

• Yang, Bing

Chair dc:contributor.committeechair

• Larson, Timothy J.

Committee members dc:contributor.committeemember

• Niehaus, Walter G. Jr.
• Sitz, Thomas O.
• Gregory, Eugene M.
• Boyle, Stephen M.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-10042006-143914

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/39623