Studies on mechanisms involved in the induction of microsomal benzpyrene hydroxylase following pretreatment with 3,4-benzpyrene anddaflatoxin BO

Microsomes obtained from different groups or rats, following pretreatment with different dosages or 3,4-benzpyrene (BP), aflatoxin Bl (Bl), ethionine (ET) and/or a combination of these, were used to obtain initial velocity data for BP disappearance. The values for Km, Vmax and K/v for different groups were obtained by the computer analysis of initial velocity data. Vmax and Km values were used to assess the quantitative and qualitative changes of microsomal B:2 hydroxylase, respectively, caused pretreatment. BP pretreatment greatly enhanced the V and also decreased the Km values. The increase in Vmax paralleled the increase in the dosage of BP from BP(lX) to BP(3X). When ET, a protein inhibitor, was given in conjunction with BP it blocked the increase in Vmax but did not block the effect of BP on the Km. The effect of BP on the qualitative properties (Km) of enzyme was observed to be a slow time dependent phenomenon which required the simultaneous presence of both the enzyme and the inducer.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Biochemistry and Nutrition

Department dc:contributor.department

Biochemistry and Nutrition

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1968

Author dc:creator

• Gurtoo, Hira Lal

Chair dc:contributor.committeechair

• Campbell, T. Colin

Committee members dc:contributor.committeemember

• Webb, Ryland E.
• Ackerman, C. J.
• Plowman, Kent M.
• Schmidt, Robert R.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-06022010-020342

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/37933