Virginia Tech
Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Abstract
dc:description.abstractTraditional serologic assays of Actinobacillus pleuropeumoniae often have problems with cross-reactivity. To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved capsular export region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the exception of serotype 4. A second set of primers specific to the unique capsular biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5 only. The sensitivity of this assay was determined to be less than 100 colony forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively distinguishes serotype 5 strains from other serotyes.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- masters
- Discipline thesis:degree_discipline
- Veterinary Medical Sciences
- Department dc:contributor.department
- Veterinary Medical Sciences
- Grantor dc:publisher
- Virginia Tech
- Year dc:date.issued
- 1997
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Lo, Terry
- Chair dc:contributor.committeechair
-
- Inzana, Thomas J.
- Committee members dc:contributor.committeemember
-
- Wong, Eric A.
- Sriranganathan, Nammalwar
Subjects
dc:subject × 4Rights
dc:rights- Statement dc:rights
-
- In Copyright
- Licence dc:rights.uri
Identifiers
dc:identifier.*- Dc Identifier Other
- etd-71497-17302
- OAI identifier oai:identifier
- oai:vtechworks.lib.vt.edu:10919/36902