The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa

A series of in vitro studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation period. However, the viability of each of the four treatments was lower than that of the unencapsulated control (p<.05 and p<.01; percentage motility and percentage intact acrosomes, respectively), indicating spermatozoal damage occurred during the encapsulation process. Capsule wall thickness and integrity for the .15%, .25%, and .35% concentrations were greater (p<.Ol) than that of the .05% capsules.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Dairy Science

Department dc:contributor.department

Dairy Science

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1984

Author dc:creator

• Fultz, Stanley Wakefield

Chair dc:contributor.committeechair

• Saacke, Richard G.

Committee members dc:contributor.committeemember

• Pearson, Ronald E.
• Akers, Robert Michael
• Keenan, Thomas W.
• White, John M.

• spermatoza viability

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-07292013-082943

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/34249