The Isolation of Natural Products From Plant Extracts

Bioassay-guided fractionation of the ethyl acetate extract of Virola sp. (Myristicaceae) using the Sc7 yeast strain resulted in the isolation of the weakly cytotoxic biochanin A (an isoflavone compound). The bioassay of three mutant yeast strains (1138, 1104, and 1353) directed the isolation of DNA-damaging alkaloids from Solanum hostmannii. These alkaloids were identified as verazine and (20R) epimer verazine. In addition, three oxoaporphine alkaloids were isolated from the bark of Papualthia sp. (Annonaceae). Oxocrebanine and atherospermidine showed DNA-damaging activity whereas liriodenine had cytotoxic activity.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Chemistry

Department dc:contributor.department

Chemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2000

Author dc:creator

• Pung, Thitiya

Chair dc:contributor.committeechair

• Kingston, David G. I.

Committee members dc:contributor.committeemember

• Hanson, Brian E.
• Tanko, James M.
• Gandour, Richard D.

• oxoaporphine
• verazines
• natural product
• biochanin A
• DNA-damaging agents

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-07122000-16230006

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/33973