Impact of a Barrier on Transport of Particles and Aerosolization of Viruses at a Wastewater Treatment Plant

Airborne microorganisms, such as bacteria, viruses and fungi, are abundant in the natural and built environments. This research encompasses two studies on virus aerosolization and transport in the built environment and the subsequent implications for human health. In the first study, we quantified the impact of a barrier on the spatial distribution of different-sized particles released by speaking in a poorly ventilated room. The room was outfitted with 108 passive sampling sites. The barrier resulted in an increase in 0.5 µm particles deposited on the source-side of the barrier and an increase in 0.5 µm particles at other locations 4-6 m from the source. The barrier had minor impacts on the distribution of 1, 6, 10 and 20 µm particles. The results from this study indicated that barriers may not serve as adequate protection to others in the room, depending on their locations relative to the barrier and the timescale of exposure. In the second study, we reviewed the applications of next-generation sequencing for viruses in water environments. We also characterized the occurrence of two viruses (crAssphage and SARS-CoV-2) from a local wastewater treatment plant (WWTP) in both water and air samples at two locations within the WWTP (influent and aeration basin). We found that crAssphage, a fecal indicator, was quantifiable in most air and water samples, but was not detected in control samples. SARS-CoV-2 N2 RNA was detected in a fraction of the water and air samples but was present in the control water samples, so results for this virus are confounded by laboratory contamination. We also found that there was no correlation between airborne and waterborne SARS-CoV-2 concentrations at the WWTP. A quantitative microbial risk assessment model was constructed to determine inhalation risks associated with airborne SARS-CoV-2 for WWTP operators. The probability of infection ranged from about 2.4 x 10-4 to 5.6 x 10-8 and was heavily dependent on exposure time, airborne concentration and other parameters.

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Civil Engineering

Department dc:contributor.department

Civil and Environmental Engineering

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2023

Author dc:creator

• Gnegy-Spencer, Mariah Ann

Chairs dc:contributor.committeechair

• Marr, Linsey C.
• Pruden, Amy

Committee members dc:contributor.committeemember

• Hamilton, Kerry
• Wigginton, Krista Rule
• Vikesland, Peter J.

• Aerosol
• virus
• barrier
• wastewater treatment
• exposure

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

vt_gsexam:36578

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/113989