A Novel Phytoestrogen that Acts as an Agonist for Human Estrogen Receptors.

Estrogen is the natural agonist of the estrogen receptor (ER). However, certain plant-derived compounds or phytoestrogens have been identified that mimic estrogens and act as agonists and/or antagonists of ERs, depending on subtype and target tissue. Understanding how phytoestrogens interact with ERs, and therefore effect the estrogenic response, may prove beneficial in hormone replacement therapy and in the prevention and treatment of hormone-related diseases. Using Thin Layer Chromatography, gas chromatography/mass spectrometry (GC/MS), and proton nuclear nagnetic resonance (HNMR), I identified 4-ethoxymethylphenol (4EM) found in Maclura pomifera. While most phytoestrogens are heterocyclic compounds, 4EM is a simple phenol that acts as an agonist of ER-alpha and -beta in HeLa and MCF-7 cells. To study the effect of 4EM on ER-alpha and -beta activity, I performed transient transfection assays and showed that 4EM activates ER dependent gene transcription in a dose dependent manner in both ER subtypes. Further, 4EM- mediated transcription in ER-alpha, like estrogen, was enhance in the presense of co-activators, SRC-1 (steroid receptor coactivator-1), CBP (CREB binding proteins), and E6-AP (E6-associated protein) and inhibited by trans-4- hydroxytamoxifen (4HT). I found that 4EM was specific for ER and did not activate transcription of the progesterone receptor in HeLa cells.

Grantor dc:publisher

University of North Texas

Year dc:date

2001

Author dc:creator

• Pearce, Virginia

Contributors dc:contributor

• Smith, Donald Wiley
• Nawaz, Zafar
• White, Olivia
• O'Donovan, Gerard A.
• Waller, William T.

• Phytoestrogens.
• Estrogen -- Agonists.
• Estrogen -- Receptors.
• estrogen
• Plant-derived compounds
• phytoestrogens
• agonists
• antagonists

Statement dc:rights

• Use restricted to UNT Community
• Copyright
• Pearce, Virginia
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 51959428
https://digital.library.unt.edu/ark:/67531/metadc3003/
ark: ark:/67531/metadc3003

OAI identifier oai:identifier

info:ark/67531/metadc3003