Characterization of the Fidelity Mechanisms of Leucyl -Trna Synthetases From Saccharomyces Cerevisiae and Escherichia Coli

We tested several mutations in yeast mitochondrial LeuRS that altered the post-transfer editing function of LeuRSs from other origins. Our results show that yeast mitochondrial LeuRS has maintained a competent editing active site for post-transfer editing of mischarged tRNA similar to other LeuRSs. However, unlike other origins, when LeuRS amino acid editing-defective mutants were introduced in vivo, cell viability was largely unaffected. In contrast, these editing-defective mutations limited viability of E. coli cells. We propose that the yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Karkhanis, Vrajesh Anil

Contributors dc:contributor

• Martinis, Susan A.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3269936

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84840