University of Illinois at Urbana-Champaign
Metabolism of Two Model Xenobiotics in Cultured Plant Cells
Abstract
dc:descriptionPlant cells in culture were found to oxygenate two model xenobiotics. Para-chlorophenyl methyl sulfide was S-oxidized by carrot, cotton, tobacco, alfalfa, and blacknightshade cell cultures. The rates of S-oxygenation by cultured plant cells were comparable to the rates in animal tissue preparations. The plant cell metabolism of the promutagen-procarcinogen 2-aminofluorene was evaluated by a mutagen bioassay. The mutagenic activation of 2-aminofluorene by cultured plant cells was detected by the induction of reverse mutation at the his locus of Salmonella typhumerium. A photoautotrophic cotton cell culture was developed to facilitate xenobiotic metabolism research in photosynthetic cultured plant cells.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Agronomy
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Blair, Louis Curtis
Subjects
dc:subject × 1Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8324510
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/71582