Regulation of Estrogen Biosynthesis in the Domestic Hen

The focus of this dissertation was a definition of the mechanism(s) which cause(s) suppression of estradiol (E(,2)) biosynthesis during follicular maturation in the domestic hen. Theca and granulosa layers from the largest (F(,1)), second largest (F(,2)), and third largest (F(,3)) follicles were collected 24, 18, 12, 6 and 2 h before ovulation and isolated cells prepared. Five experiments were done: (1) characterization of optimal conditions for E(,2) secretion by theca cells; (2) measurement of E(,2) secretion by F(,2) and F(,3) theca cells; (3) measurement of androgen production by F(,1), F(,2), and F(,3) granulosa and theca cells; (4) measurement of estrogen production by F(,1), F(,2), and F(,3) granulosa and theca cells; and (5) evaluation of the effect of exogenous progesterone (P(,4)), testosterone (T) and E(,2) secretion by F(,3) theca cells.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Wang, Shiow-Ching

• Agriculture, Animal Culture and Nutrition

Identifier

(UMI)AAI8218581

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/69997