Photochemical Studies of Rhodopsin, Purple Membrane, and Their Analogues

Rhodopsin and analogues. The absorption spectrum of the bathoproduct of rhodopsin, which contains 11-cis retinal as chromophore, is obtained at 77(DEGREES) K under the condition that on the average less than one photon can be absorbed by each rhodopsin molecule. The spectrum, with (lamda)(,max) at 542 nm, is found to be almost identical to the spectrum obtained by Yoshizawa and Wald (1963) from measurements on photostationary state mixtures. In addition, absorption spectra are also measured for the bathoproducts of two rhodopsin analogues the chromophores of which are two different isomers of retinal, isorhodopsin I with 9-cis retinal and isorhodopsin II with 9,13-dicis retinal. The bathoproduct of isorhodopsin I is identical to that of rhodopsin within experimental errors, while the spectrum for the bathoproduct of isorhodopsin II is different. The photochemistry of the dicis pigment appears to be different from rhodopsin and isorhodopsin I.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Mao, Boryeu

• Biophysics, General

Language dc:language

eng

Identifier

(UMI)AAI8018181

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67594