Enzymatic diversity in lipoic acid modification of proteins

Escherichia coli is one of a few organisms with a well characterized and complete metabolic model for lipoic acid metabolism. In this thesis I make discoveries through analysis of the diversity of lipoic acid metabolic genes in other organisms. By examining the distribution and phylogeny of the lipoate ligase protein family, I and others have found gaps and inconsistencies in our knowledge of lipoic acid metabolic pathways. I examine select proteins through heterologous expression in E. coli, allowing complementation studies and biochemical analysis. This enables prediction of lipoic acid metabolism in various organisms and reveals novel pathways and enzymology.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Christensen, Quin H.

Contributors dc:contributor

• Cronan, John E.
• Imlay, James A.
• Metcalf, William W.
• Olsen, Gary J.

• lipoic acid
• amidotransfer
• Bacillus
• Thermoplasma

Statement dc:rights

• Copyright 2011 Quin H. Christensen

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/24498

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/24498