A study of Escherichia coli integration host factor interaction with DNA

The sequence elements required for IHF binding to the H$\sp\prime$ and H1 sites in attP of $\lambda$ were examined. The H$\sp\prime$ site naturally contains a dA+dT element 5$\sp\prime$ to the core consensus element WATCAANNNNTTR, while the H1 site does not. It was found that both elements are required for IHF to bind to the H$\sp\prime$ site. In contrast, the core consensus determinant alone is sufficient for IHF binding to the H1 site. Placement of a dA+dT element upstream of the H1 core consensus element significantly increased the affinity, suggesting that the presence of a dA+dT element enhances IHF binding.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Hales, Laura Marie

Contributors dc:contributor

• Gardner, Jeffrey F.

• Biology, Molecular
• Biology, Microbiology

Statement dc:rights

• Copyright 1995 Hales, Laura Marie

Language dc:language

eng

Identifier

AAI9624356
(UMI)AAI9624356

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19066