Partial Characterization of Animal Growth Inhibitors from Soybeans

<p>The pH 4. 4 supernatant from raw (unheated) soybean meal was separated into 5 fractions on a Sephadex G-25 column in efforts to isolate the animal growth inhibitors present soybeans. Each fraction was added to the diet of growing mice and growth inhibition (GI) activity was determined by comparing their_ growth rates to g1"'owth rates achieved on an autoclaved soybean meal diet. Fractions I and II each contained about 50% of the GI activity, with a trace in fraction III and none in fractions IV and V. Only fraction I contained trypsin inhibitor and caused pancreatic enlargement. Fraction I was further separated into 2 fractions (Ia and Ib) on a Sephadex G-50 column. Fraction Ib contained trypsin inhibitor and caused pancreatic enlargement. Both fractions Ia and Ib contained GI activity, with most of the GI activity present in Ib. Fraction II was further separated into 2 fractions (IIa and IIb) on a phosphocellulose column eluted with a KCl gradient. Most of the GI activity was present in fraction IIb. Amino acid analysis of G-25 - II indicated all amino acids present, with the sulfur-containing amino acids, methionine and half cystine, present in only very small amount.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis - Open Access

Discipline thesis:degree_discipline

Dairy Science

Year dc:date.available

1973

Author dc:creator

• Uckert, Joseph Richard

• Dairy Science

Statement dc:rights

• <p>In Copyright - Non-Commercial Use Permitted<br /><a href="http://rightsstatements.org/vocab/InC-NC/1.0/">http://rightsstatements.org/vocab/InC-NC/1.0/</a></p>

Language dc:language

en

Repository record dc:identifier

https://openprairie.sdstate.edu/etd/1279

OAI identifier oai:identifier

oai:openprairie.sdstate.edu:etd-2280