Understanding the Effect of BVDV on Innate Immune Response of Neutrophils

<p>Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. The current study measured the effect of BVDV infection on viability of neutrophils, their surface marker expression and functional abilities including migration/chemoattraction, phagocytosis, reactive oxygen species production (oxidative burst) and neutrophil extracellular trap (NET) formation. These studies revealed that none of BVDV strains affected the viability of neutrophils in vitro. BVDV infection did affect surface marker expression. TGAC and TGAN reduced the expression of CD18 and Lselectin while increasing CD14 expression. All ncp BVDV strains used enhanced neutrophil migration while the cp BVDV strain reduced neutrophil migration as compared to mock-infected control treatment. Among the BVDV strains used in current study, highly virulent 1373 significantly enhanced neutrophil migration. The enhancement in neutrophil migration by 1373 was approximately 55% higher as compared to LPS-treated positive control macrophages. BVDV infection significantly enhanced neutrophil phagocytosis activity for 0.2 μm microsphere beads as compared to mock infection. Neutrophil phagocytic activity for rhodamine-labeled E. coli was reduced by BVDV infection as compared to LPS-control. TGAC, TGAN, 1373 or 28508 had 23%, 6%, 19% or 12% less phagocytic activity respectively as compared to mock-infected rhodamine-labeled E. coli-treated neutrophils. All BVDV strains used in the current study also reduced oxidative burst by approximately 50% as compared to positive control (p</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis - Open Access

Discipline thesis:degree_discipline

Veterinary and Biomedical Sciences

Year dc:date.available

2017

Author dc:creator

• Thakur, Neelu Singh

Contributors dc:contributor

• Christopher C. L. Chase

• Bovine viral diarrhea virus
• immunosuppression
• innate immune response
• neutrophil extracellular trap
• Neutrophils
• oxidative burst
• Animal Diseases
• Veterinary Infectious Diseases
• Veterinary Microbiology and Immunobiology

Licence

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

en

Repository record dc:identifier

https://openprairie.sdstate.edu/etd/1235

OAI identifier oai:identifier

oai:openprairie.sdstate.edu:etd-2236