Development and Characterization of A Recombinant Orf Virus Vector Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus

<p>Orf virus (ORFV), the type member of the genus <em>Parapoxvirus</em> of the family <em>Poxviridae</em>, causes orf or cutaneous pustular dermatitis in sheep and goats. ORFV is a ubiquitous virus capable of re-infecting its hosts multiple times over time. ORFV causes a non-systemic, self-limiting disease which is usually restricted to the skin surrounding the virus entry sites. ORFV has evolved several immunomodulatory proteins (IMPs) that evade and/or modulate host immune responses to infection and contribute to virus virulence and disease pathogenesis. Given biological properties and unique immunomodula tor y properties, ORFV has gained significant attention in recent years for its potential as a vaccine delivery vector for use in veterinary medicine. Here we explored the potential of ORFV as a vaccine delivery vector for use in swine. The spike (S) protein of porcine epidemic diarrhea virus (PEDV) was used as our model antigen and the immunogenicity and protective efficacy of a recombinant ORFV expressing the full length S protein of PEDV (ORFV-PEDV-S) was assessed in pigs. The ORFV-PEDV-S virus was generated by homologous recombination and the DNA sequences of PEDV S were inserted into the ORFV121 gene locus, an immunomodulatory protein (IMP) encoded by ORFV that contributes to the virus virulence. The resultant recombinant virus was characterized <em>in vitro</em> and its immunogenicity and protective efficacy evaluated <em>in vivo</em>. Results of our study show that intramuscular (IM) immunization of swine with the ORF-PEDV-S recombinant virus elicited robust humoral immune responses and protected animals from clinica l disease. Additionally, IM immunization with the ORFV-PEDV-S led to reduced virus shedding in feces. The results of this study provide important information on the feasibility of ORFV as a vaccine delivery vector for use in swine.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis - Open Access

Discipline thesis:degree_discipline

Veterinary and Biomedical Sciences

Year dc:date.available

2016

Author dc:creator

• Hain, Kyle

Contributors dc:contributor

• Diego G. Diel

• Orf
• parapoxvirus
• PEDV
• vaccine
• vector
• virus
• Biology
• Microbiology
• Veterinary Microbiology and Immunobiology
• Virology

Licence

• http://rightsstatements.org/vocab/InC/1.0/

Repository record dc:identifier

https://openprairie.sdstate.edu/etd/1151

OAI identifier oai:identifier

oai:openprairie.sdstate.edu:etd-2152