Estudio de la penetración de agente antiinfecciosos en macrófagos utilizando eritrocitos portadores

[EN] The first objective of this study was to perform a complete review about the problems in the treatment of intracellular infections. It also reflects the different types of delivery systems currently available and the benefits they bring in the treatment of these infections, as they facilitate access antiinfeciosos agents into the cell. The second objective of this work has been making point and validation of analytical techniques that allow quantification of the drugs studied in different types of biological samples such as erythrocytes, macrophages, plasma and tissues. They have perfected a technique of HPLC-fluorescence for the quantification of amikacin, a technique of HPLC-UV for quantification of zidovudine in red blood cells and a technique for quantifying UPLC-MS/MS zidovudine in plasma and tissues. The third objective of this work has been the development and optimization of methods for the encapsulation in erythrocytes of rats of selected drugs. For the encapsulation of amikacin has been used a hypotonic dialysis technique has been set-up previously in our laboratory. Subsequently, this technique has been optimized for encapsulation of the antiretroviral zidovudine. To do this we have studied the influence of different factors including the initial amount of drug, time on dialysis, and dialysis volume ratio / hypotonic buffer in both the encapsulation efficiency and in hematological properties of erythrocytes. Finally, fourth objective of this work was to study the penetration of amikacin and zidovudine in macrophages both in vitro and in vivo, as well as changes in the pharmacokinetics and tissue distribution of these drugs in rats when administered by carriers incorporated into erythrocytes intraperitoneally, compared with its use in free form.

Author

• Briones Cuesta, Elsa

• Tesis y disertaciones académicas
• Universidad de Salamanca (España)
• Academic dissertations
• Agentes antiinfecciosos
• Anti-infective agents
• Eritrocitos
• Erythrocytes
• Macrófagos
• Macrophages
• Pharmacology

Identifier

hdl:10366/76416

OAI identifier oai:identifier

oai:gredos.usal.es:10366/76416