Studies on the Production of γM and γG Antibodies in the Mouse

<p>The organ localization of intravenously administered C¹⁴-labeled intact S. adelaide was studied in mice. In the absence of circulating antibody, the spleen had a higher specific activity than other organs studied. The primary antibody response to this antigen was then studied using a specific flagellar immobilization assay. Spleen homogenate supernatants exhibited two distinct antibody peaks which were well separated in time. The serum titer curve was not detectably bimodal. Sera and spleen homogenate supernatants were characterized by electrophoresis, ultracentrifugation, and treatment with 2-mercaptoethanol. The primary antibody response was found to consist of the early, transient production of γM antibodies followed by the production of γG antibodies. This response was quantitatively reduced but not qualitatively affected by the removal of spleens from the mice before antigen injection. Methotrexate and colchicine both exhibited differential effects on the synthesis of γM and γG when they were administered during the antibody response. With certain doses of each, the γG response seemed more susceptible to inhibition. In all cases, however, the γG response recovered to near normal levels after a delay. Histological studies during a methotrexate inhibited response showed that normal levels of γM could occur in the serum at a time when there were no pyroninophilic blast cells or secondary nodules in the spleen. The recovery of γG was well correlated with the appearance of many haemocytoblasts in the red pulp. At that time there were still no secondary nodules in the white pulp. γG antibodies recovered to nearly normal levels despite the fact that maturation of the haemocytoblasts to mature plasma cells was inhibited. A study was made of the transfer of spleen cells from primed animals to homologous X-irradiated recipients. When spleen cells were taken from immunized donors on day 2 or day 6 after injection of antigen, only γM antibody could be demonstrated in the recipients. Spleen cells taken on day 8 or day 10 after antigen produced mostly γG antibody in the recipients.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Thesis

Year

1965

Author dc:creator

• Blinkoff, Richard Charles

Contributors dc:contributor

• Zanvil Cohn

• S. adelaide
• antibody response
• γM and γG antibodies
• spleen cells
• methotrexate inhibition
• immunoglobulin class switching
• Life Sciences

Repository record dc:identifier

https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/601

OAI identifier oai:identifier

oai:digitalcommons.rockefeller.edu:student_theses_and_dissertations-1607