A bioactive peptide QUB-1370 from the frog skin secretion of Pelophylax nigromaculata

Natural products are sources for the development of novel drugs to treat human diseases. Bioactive peptides from amphibian skin secretions have been demonstrated to have multiple biological activities, such as antimicrobial activity, anticancer activity and so on. Furthermore, some of the peptides have been proven as the promising candidates of new drugs.<br/>A cDNA precursor encoding the peptide was identified from the skin secretion of Pelophylax nigromaculata by using molecular cloning. The mature peptide was named QUB-1370 according to its molecular mass. The sequence is: VIPIVSGLLFSLL-NH2. Then the peptide synthesizer was used to synthesis the peptide and the peptide was purified by using RP-HPLC. To test the bioactivities of QUB-1370, a series assays such as antimicrobial assay, anticancer assay and haemolysis examinations were performed. However, the results showed that QUB-1370 didn't show any biological functions in these experiments. <br/>

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2018

Author dc:creator

• Wu, Ying

Advisors dc:contributor.advisor

• Zhou, Mei
• Wang, Lei

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/75118ab4-d809-4add-a7f4-37c092d94654

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/75118ab4-d809-4add-a7f4-37c092d94654