Characterized analysis of peptide QUB-1433 from the skin secretion of the dark-spotted frog (Pelophylax nigromaculatus)

Bioactive peptide isolated from the granular glands of frog skin have been widely discovered to exhibit antimicrobial and anticancer activities. Since the antibiotic resistance has become a severe difficulty in modern therapeutic treatment, many of them have been developed into clinical application for the treatment of microbial infection and cancer.<br/><br/>In this thesis, a potent natural antimicrobial peptide QUB-1433 was identified and cloned from the skin secretion of Pelophylax nigromaculatus via “shotgun” cloning. According to the amino acid residues of QUB-1433, the pure peptide was synthesised and purified via solid-phase peptide synthesis and RP-HPLC. Subsequently, antimicrobial, anticancer and haemolysis assays were performed on QUB-1433 for its functional evaluation. From the results of a series of bioactive assays, QUB-1433 displayed activities of against Gram-positive bacteria and fungi (MIC values are 16 and 32 µM), but is lack in broad-spectrum of anticancer ability (MIC value is 128 µM). Moreover, QUB-1433 possesses relative low cytotoxicity towards mammalian cell. Hence the bioactivities of QUB-1433 provided new insight in the discovery of new drugs. However, the mechanisms of action and optimization of QUB-1433 still required the further exploration. <br/><br/><i>Thesis embargoed until 31st October 2024 </i>

Name dc:type.qualificationname

Master of Philosophy

Level dc:type.qualificationlevel

Masters Thesis

Grantor dc:publisher.institution

Queen's University Belfast

Year dc:date.issued

2019

Author dc:creator

• Wang, Wenjie

Advisors dc:contributor.advisor

• Chen, Tianbao
• Wang, Lei
• Zhou, Mei
• Ma, Chengbang

Language dc:language

eng

Identifier

oai:pure.qub.ac.uk/portal:studenttheses/0de41f07-2a57-49cb-a6a8-19545ae90b49

OAI identifier oai:identifier

oai:pure.qub.ac.uk/portal:studenttheses/0de41f07-2a57-49cb-a6a8-19545ae90b49