Analysis of peak characterization and data alignment of a differential mobility spectrometer for the discovery of TB biomarkers in human breath

Biomarkers have become a growing field for disease research and diagnostics. For the infectious, deadly disease, tuberculosis (TB), the discovery of effective TB biomarkers in the human breath may enable the development of a cheap, fast, and accurate TB diagnostic to fight against this epidemic. The Charles Stark Draper Laboratory is employing a differential mobility spectrometer (DMS), in order to identify key biomarkers for TB. Biomarker candidates appear as peaks in the DMS sensor data at particular retention times and DC compensation voltages. Possible biomarkers are identified through well-known classification techniques, K-nearest neighbors and support vector machines. This research is focused on the development of a toolbox that will aid in the discovery and in-depth understanding of the TB breath biomarkers. This thesis demonstrates the algorithms and capabilities of peak quantification, characterization, RIP-based alignment, and siloxane-based alignment on protein and bacterial sensor data, including those from initial Mycobacterium tuberculosis samples. The results show that siloxane-based alignment with high-intensity peak landmarks effectively increase DMS peak stabilities and signal-to-noise ratios. These new tools will be important for the analysis of specific TB biomarker candidates as our group at the Charles Stark Draper Laboratory receive more samples of M. tuberculosis.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2008

Author dc:creator

• Tang, Amy Yuen-Lee

Advisor dc:contributor.advisor

• Roger G. Mark and Nirmal Keshava.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/46526

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/46526