Quantitative biological Raman spectroscopy for non-invasive blood analysis

The long term goal of this project is the measurement of clinically-relevant analytes in the blood tissue matrix of human subjects using near-infrared Raman spectroscopy, with the shorter term research directed towards glucose measurements for diabetic patients. This optical technique enables non-contact, painless measurements with no sample preparation and simultaneous determination of multiple analytes. Such a technology could greatly impact the healthcare practices for the entire population. This thesis presents advances in quantitative biological Raman spectroscopy along three avenues: instrument optimization, analyte-specific information extraction, and correction for sampling volume variations. In the first category, we have built a high-throughput instrument that integrates Raman and diffuse reflectance capabilities. Additionally, new algorithms have been developed to enhance wavelength precision and stability. Using this instrument, we have presented evidence of glucose-specific measurements in human and dog subjects. We believe that this is the first time glucose-specific information is extracted transcutaneously in vivo using Raman spectroscopy.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2007

Author dc:creator

• Shih, Wei-Chuan

Advisor dc:contributor.advisor

• Michael S. Feld.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/40362

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/40362