Characterization of nanofilter arrays for small molecule separation

Experimental studies were performed to evaluate methods of improving separation resolution and speed in microfabricated nanofilter arrays. Experiment parameters investigated include electric field strength, nanofilter geometry, and buffer concentration. DNA polymers of size 25-1000 base pairs were the subject of our study. We concluded that increasing electric field strength resulted in inferior separation for larger DNA polymers (400-1000 bp). Additionally, we quantified the improvement in resolution of smaller nanofilter pores and lower buffer concentration. A theoretical model based on Macrotransport Theory was developed to estimate average species velocity and peak dispersion.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2006

Author dc:creator

• Bow, Hansen Chang

Advisor dc:contributor.advisor

• Jongyoon Han.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/37934

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/37934