Algorithms for simulating human pre-mRNA splicing decisions

In this thesis, I developed a program, ExonScan, to simulate constitutive human pre-mRNA splicing. ExonScan includes several models for splicing components, including splice sites, exonic splicing enhancers, exonic splicing silencers, and intronic splicing enhancers. I used ExonScan to test various aspects of human splicing, including correlation of splicing signal strength with tissue expression levels, the effectiveness of experimentally determined exonic splicing silencers, and splice site identification.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Rolish, Michael E. (Michael Edward)

Advisor dc:contributor.advisor

• Christopher B. Burge.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/37066

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/37066