Genome sequencing technology : improvement of the electrophoretic sequencing process and analysis of the sequencing tool industry

A primary bottleneck in DNA-sequencing operations is the capacity of the detection process. Although today's capillary electrophoresis DNA sequencers are faster, more sensitive, and more reliable than their precursors, high purchasing and running costs still make them a limiting factor in most laboratories like those of the Broad Institute. It is important to run those sequencers as efficiently as possible to reduce costs while producing robust assemblies. Polymer media for electrophoresis is the most important determinant for sequencing throughput. This thesis investigates the effect of polymer media on the performance of Applied Biosystems (ABI) 3730xl, the de-facto standard of DNA sequencers and develops analysis procedures for ABI3730xl system and its data. Due to its use in the human genome project (HGP), ABI has established a monopolistic position in the DNA-sequencing tool industry. As the de-facto standard of DNA sequencers ABI3730xl is highly automated, well-optimized, and black-boxed, despite the importance of higher throughput sequencing for diagnostic applications, third parties have found it difficult to improve sequencing methods.

Department dc:contributor.department

Leaders for Manufacturing Program at MIT

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Maruyama, Kazunori, Ph. D. Mie University

Advisor dc:contributor.advisor

• Roy E. Welsch and Patrick S. Doyle.

• Chemical Engineering.
• Sloan School of Management.
• Leaders for Manufacturing Program.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/34868

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/34868