The development of an in vitro, one-pass, high-throughput model of flow dependent thrombosis

Thrombosis is an initiating response to a vascular injury, which aids in the repair and remodeling of the vessel wall. However, if this process remains unchecked, occlusion of the arterial lumen may quickly occur. The arterial vascular bed is a delicate and life-sustaining environment, in which a pathological thrombosis can bring about devastating conclusions such as acute vascular syndromes or post-interventional thrombosis. In order to explore these flow-dependent thrombotic reactions, it is essential to consider the physical environment present inside the vasculature. A novel in vitro, high-throughput method for creating one-pass blood flows has been developed to model the arterial environment. Flow is generated in a matrix of small glass tubes with varying inner diameters through the use of a constant pressure drop. Using this technique, a variety of flow rates are created in the numerous tubes, resulting in a variety of flow shear rates. In addition, this technique allows for the monitoring of sensitive, flow-dependent processes without the disturbances from pump action and circuit effects. A detailed discussion about the goals of the proposed systems is included, as well as the methodology employed to choose the optimal flow system, and the process by which the components of the system evolved in design.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Echenique, Javier Jacobo

Advisor dc:contributor.advisor

• Elazer R. Edelman.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/32861

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/32861