Effects of the conserved proteins, Soj (ParA) and SpoOJ (ParB) on chromosome partitioning in Bacillus subtilis

The Par system contributes to stable inheritance of plasmids and chromosomes in diverse bacterial species. Faithful chromosome partitioning is important to dividing cells because anucleate, or chromosomeless, cells are not viable. Since the discovery of the Par system in low-copy number plasmids over 20 years ago, research has focused on characterizing its components: ParA, a DNA-binding ATPase, ParB, a DNA-binding protein that interacts with ParA, and parS, the site bound by ParB. How these proteins function to stabilize parS-containing plasmids or chromosomes remains to be elucidated. Less is known about the chromosomally-encoded Par systems. Intriguingly, in several organisms including Bacillus subtilis, chromosomally-encoded Par systems play important roles in cellular processes besides chromosome partitioning. This thesis focuses on characterizing the roles of Soj (ParA) and SpoOJ (ParB) of B. subtilis in chromosome partitioning. These proteins were first identified by their effects on sporulation. My results indicate that Soj and SpoOJ contribute to timely separation of replicated chromosomal origins, and argue against the model that SpoOJ anchors replicated origins to putative receptors in opposite halves of the cell. Additionally, I show that Soj and SpoOJ regulate replication of initiation, and propose that this regulation may occur by a similar genetic hierarchy through which they regulate initiation of sporulation.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2004

Author dc:creator

• Lee, Philina S. (Philina Sharlene), 1976-

Advisor dc:contributor.advisor

• Alan D. Grossman.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/32255

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/32255