Studies on the molecular mechanism of T cell triggering and detection and characterization of antigen-specific T cells

(cont.) Identification and characterization of T cell epitopes derived from infectious agents or vaccines can greatly enhance the ability to study and eventually direct the immune response. This work contains a description of a novel technique for identifying and characterizing specific T cell responses in parallel. The system involves incubating heterogeneous T cell mixtures with artificial antigen-presenting microarrays, which include immobilized cytokine capture antibodies, co-stimulatory molecules, and MHC complexes presenting many different potential T cell epitope peptides. These microarrays can rapidly, conveniently, and sensitively detect antigen-specific T cells and characterize the functional responses to many different epitopes in parallel in a location-dependent manner.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Chemistry.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2005

Author dc:creator

• Stone, Jennifer Drignat, 1976-

Advisor dc:contributor.advisor

• Lawrence J. Stern.

• Chemistry.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/27871

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/27871