Studies of the division cycle of human cancer cells (HELA) using a new system for continuous synchronization

HeLa S3 cells have been induced to divide synchronously in suspension culture through more than ten generations. Cellular DNA and RNA have been determined at hourly intervals throughout the cell cycle defining the pattern of nucleic acid accumulation in HeLa cells. Cellular activity of the enzymes thymidine kinase, alkaline DNase, catalase, acid phosphatase and lactic dehydrogenase has been determined as a function of the division cycle. The method of synchronization developed here allows the continuous synchronous growth of mammalian cells in suspension culture. This system facilitates studies of cell cycle events since it provides a large amount of cellular material with minimal maintenance time required.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Nutrition and Food Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

1971

Author dc:creator

• Thilly, William George.

Advisor dc:contributor.advisor

• Gerald N. Wogan.

• Nutrition and Food Science.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/148422

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/148422