Experimental investigations of isotopologue fractionation during microbial methanogenesis

The work described in this thesis explores and develops different culturing methods to test the following hypothesis: hydrogen concentration and redox potential are important controlling factors of carbon (¹³C/¹²C) and hydrogen (D/H) isotope ratios as well as the abundance of methane clumped isotopologues ([delta]¹³CH₃D) during microbial methanogenesis. Chapter 2 uses batch and fed-batch culturing systems to investigate the effects of H₂ concentrations on isotopologue fractionation. The results from fed-batch experiments confirmed the previous observation of decoupled ¹³C/¹²C and D/H systematics and provide experimental support for the hypothesis linking D/H and [delta]¹³CH₃D systematics.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Rhim, Jeemin Hannah.

Advisor dc:contributor.advisor

• Shuhei Ono.

• Earth, Atmospheric, and Planetary Sciences.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/129057

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/129057