Surface plasmon enhanced fluorescence for biological imaging : from visible to short-wave infrared

Fluorescence imaging offers high spatio-temporal resolution, low radiation dosage exposure, and low cost among all the available imaging modalities, for example, magnetic resonance imaging, computerized tomography and positron emission tomography. Imaging probes of high emissivity and photostability are the key to achieving fluorescence imaging with high signal-to-background ratio (SBR). One promising approach to developing highly bright and stable imaging probes is through surface plasmon enhanced fluorescence. In the first part of the thesis, we develop a fluorescent probe with high site-specificity and emission efficiency by exploiting the targeting-specificity of M13 virus and co-assembling plasmonic nanoparticles and visible dye molecules on the viral capsid. Practical factors controlling fluorescence enhancement, such as nanoparticle size and dye-to-nanoparticle distance, are studied in this project. Lastly, the highly fluorescent probe is applied for in vitro staining of E.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Materials Science and Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Huang, Shengnan,Ph.D.Massachusetts Institute of Technology.

Advisor dc:contributor.advisor

• Angela M. Belcher.

• Materials Science and Engineering.

Statement dc:rights

• MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/129028

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/129028