Design and debugging of ultrastable engineered genetic systems

Engineered genetic systems in bacteria have tremendous potential for biotech applications ranging from living therapeutics to the controlled production of chemicals. Engineering such genetic systems is challenging as these genetic systems often consist of multiple genes and genetic parts (>4 genes or > 45 genetic parts) interacting with each other as intertwined networks. These intertwined networks are invisible, making the design and debugging of these genetic systems to be particularly challenging. Additionally, expressing a large number of genes creates burdens on the host cell and reduces the long-term stability of these genetic systems. Here we address these two problems by (i) adapting high-throughput RNA-seq to visualize the inner-workings of these engineered genetic systems and (ii) developing a robust and efficient genome engineering platform that enables the implementation of long-term stable engineered genetic systems on the genome.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Biological Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Park, Yongjin,Ph. D.Massachusetts Institute of Technology.

Advisor dc:contributor.advisor

• Christopher Voigt.

• Biological Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/124182

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/124182