Biosensor-based strategies for improving pathway production in Escherichia coli

Microbial production of chemicals and fuels is an attractive renewable alternative to petroleum-based processes. D-glucaric acid, a Department of Energy top value-added chemical from biomass, is a precursor to polymers such as nylons and used in detergents. An engineered metabolic pathway requiring three heterologous enzymes to convert glucose into glucaric acid in Escherichia coli was previously demonstrated by the Prather lab. Glucaric acid production has been shown to be limited by the two downstream enzymes myo-inositol-1-phosphate synthase (MIPS) and myo-inositol oxygenase (MIOX). This work develops and deploys a biosensor that recognizes a pathway intermediate in order to overcome both limitations. A biosensor for myo-inositol (MI) was developed using the transcriptional regulator IpsA from the organism Corynebacterium glutamicum. A hybrid promoter was designed to enable function in the desired host organism E. coli.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemical Engineering

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Doong, Stephanie J.

Advisor dc:contributor.advisor

• Kristala L. J. Prather.

• Chemical Engineering.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/123072

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/123072