Contribution à l'étude de la production d'hydrogène par voie microbiologique

Hydrogen is currently considered as to become an important energy carrier and energy resource in the next century. Research and developments are now focusing on a green way to produce the hydrogen. One of these way is the microbial way, which uses microorganisms in anaerobic condition to produce hydrogen and volatile fatty acids in aqueous media. This technology may also work with liquid wastes treatment coming from the food industry. Chimiotrophic bacterias from the genus Clostridium shows many advantages due to the substrates divsersity they can assimilate but also to the production yields they reach. The study of hydrogen production by Colstridium butyricum CWBI sp1009, isolated and characterized in earlier works in the CWBI, is the main purpose of this work. This strain was inoculated in a continuous anaerobic bio reactor with fixed biomass on a rotative horizontal cylinder. This reactor reach a glucose conversion yield of 2.1 mol_hydrogen/mol_glucose consumed. The phenomenas occurring in this reactor need to be completely understood for a future application at an industrial scale. One factor influencing the hydrogen production without being well understood is the hydrogen partial pressure and the retro inhibition it affects upon the organisms. Increasing the hydrogen transfer from the liquid to gas phase in batch fermentation in 200ml of culture media and degassing daily with nitrogen enhance the hydrogen production yield of 33%. This has also been confirmed on the bio disk bio reactor by lowering the total pressure in the reactor of 0.11bar leading to a productivity and yield enhancement. The inter facial transfer surface play also a great role and was studied in 200ml batch fermentation. These experiences shows a 25 to 100% increase of hydrogen production yield by enhancing the mixing conditions of the media. Furthermore, these experiences shows influences of the culture conditions to the microorganisms metabolisms, observing the increasing activity of the formate hydrogen lyase enzyme, partially responsible of the hydrogen production.

Grantor dc:publisher

ULiège - Université de Liège

Year dc:date

2008

Author dc:creator

• Beckers, Laurent

Contributors dc:contributor

• Thonart, Philippe
• Centre Wallon de Biologie Industrielle (CWBI)

• biological hydrogen
• anaerobic fermentation
• waste valorization
• Life sciences
• Microbiology
• Engineering, computing & technology
• Chemical engineering
• Environmental sciences & ecology
• Sciences du vivant
• Microbiologie
• Ingénierie, informatique & technologie
• Ingénierie chimique
• Sciences de l’environnement & écologie

Language dc:language

fr

Identifier

info:hdl:2268/15685

OAI identifier oai:identifier

oai:orbi.ulg.ac.be:2268/15685