Tissue-specific gene expression and promoter characterization in triticale

Triticale (x Triticosecale Whitm.) is a cereal with favorable agronomic traits for a Canadian bioproduction platform crop. Appropriate tissue sampling times were determined and gene expression profiles were evaluated in five triticale seed tissues and eleven vegetative tissues using the Affymetrix Wheat GeneChip®. Genes that were expressed, not expressed, tissue-specific, tissue-enriched and developmentally regulated were identified. The percentage of probe sets on the wheat GeneChip with gene ontology annotations was improved from less than 3% to over 76% using homologous sequence identification and annotation transfer. This information was used to determine functions and processes over-represented within the identified gene lists and provide biological meaning to the results. Expression of candidate genes was further evaluated using qRT-PCR, RNA in situ hybridization and promoter characterization. This study has provided a comprehensive triticale gene expression atlas; knowledge regarding triticale development, gene function, expression and regulation; and tools enabling further triticale research and development.

Author

• Penniket, Carolyn Renee

• Triticale -- Genetics -- Research
• Triticale -- Biotechnology
• Gene expression -- Research
• Promoters (Genetics) -- Research
• Plant biotechnology -- Research
• Dissertations, Academic

Identifier

hdl:10133/3373

OAI identifier oai:identifier

oai:opus.uleth.ca:10133/3373