Cell line development and multitrophic aquaculture of the lined seahorse, Hippocampus erectus

Seahorses are ambassadors for conservation efforts and are being driven to extinction. Aquaculture has been proposed to contribute to sustainability solutions, but this is jeopardized by disease. A novel, SH3-fin cell line derived from Hippocampus erectus was characterized. Cell growth, quantified by alamarBlue cell viability assays, determined that SH3-fin tolerated room temperature to 31 ˚C and fetal bovine serum concentrations of 10 to 30 %. SH3-fin was permissive to growth of viral hemorrhagic septicemia virus genotype IVb and frog virus 3, as demonstrated by quantitative reverse transcriptase polymerase chain reaction and tissue culture infectious dose titers. Multitrophic recirculation aquaculture systems (MRAS) were developed to reduce disease and labour and to improve economic benefit by simulating natural environments using integration of Gracilaria, Caulerpa macroalgae, and Lysmata wurdemanni shrimp. The MRAS improved behaviour, water quality, and survival (not statistically significant), yet hindered husbandry and introduced opportunistic pests.

Grantor dc:publisher

University of Guelph

Author dc:creator

• Kecheliev, Dimo

Advisor dc:contributor.advisor

• Lumsden, John

• seahorse
• aquaculture
• multitrophic
• cell line

Statement dc:rights

• Attribution 4.0 International

Licence dc:rights.uri

• http://creativecommons.org/licenses/by/4.0/

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10214/28229

OAI record oai:identifier

https://atrium.lib.uoguelph.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Aatrium.lib.uoguelph.ca%3A10214%2F28229