Controlled Intracellular delivery of molecules using Nanoparticle-mediated Photoporation

Intracellular drug delivery has been of interest in recent years after the development of drugs targeting intracellular components. Delivery of exogenous material into cytosol has a variety of applications including cellular studies, therapies and diagnostics. Nanoparticle-mediated photoporation is novel platform technology, which enables intracellular delivery through transient cell membrane pore formation. In this thesis, we have explored the effects of changes in cellular microenvironment on cellular responses. Specifically, we highlight the role of nanoparticle and associated transient parameters, investigate cell responses to the presence of serum during photoporation and, provide operating conditions for enhanced macromolecular delivery for in vitro applications.

Level thesis:degree_level

Doctoral

Department dc:contributor.department

Chemical and Biomolecular Engineering

Grantor dc:publisher

Georgia Institute of Technology

Year dc:date.issued

2020

Author dc:creator

• Kumar, Simple

Advisor dc:contributor.advisor

• Prausnitz, Mark R.

Committee members dc:contributor.committeemember

• Thadhani, Naresh N.
• Champion, Julie
• Behrens, Sven H.
• Emelianov, Stanislav

• Intracellular delivery
• Carbon black, Nanoparticle
• Photoporation
• Macromolecules

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/1853/64962

OAI identifier oai:identifier

oai:repository.gatech.edu:1853/64962