Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.

<p>Group II Introns are small segments of DNA that reside in the chromosome of bacteria or the organelles of primitive eukaryotes. These elements have some very interesting properties. First, they are retrotransposons that can move from one location to a new location in DNA via a reverse transcription mechanism. Second, they form a large ribozyme that mediates self-splicing of the intron from pre-mRNA. A Group II Intron type protein with similarity to reverse transcriptase was discovered in the thermophilic bacterium <em>Geobacillus stearothermophilus</em> strain 10 (Vellore <em>et al.</em>, 2004, Appl. Environ. Microbiol. <em>70</em>: 7140-7147). Numerous copies of the intron, designated Gs. Int1, are present in the chromosome of strain 10 but absent from a related strain ATCC 12980. Experiments to detect the <em>in vivo</em> splicing of intron Gs.Int1 from <em>G. stearothermophilus</em> cells did not work. Plasmids to that will over-express the Gs. Int1 intron to detest splicing <em>in vivo</em> in <em>Escherichia coli</em> have been constructed.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2007

Author dc:creator

• Sun, Huijing

• Group II Intron
• Geobacillus stearothermophilus
• Thermophilic bacterium
• Genetics and Genomics
• Life Sciences
• Molecular Genetics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/2026

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3387