Studies of Surfactants Effect on Riboflavin Fluorescence and Its Determination in Commercial Food Products and Vitamin Tablets.

<p>A simple and economical fluorometer using blue LEDs excitation sources and simple PMT detection had been built, assembled, optimized, and employed for measurement of fluorescence from riboflavin (vitamin B₂). Surfactants have been known to enhance the intensity of fluorescence of fluorescent compounds. Fluorescence analysis of riboflavin in the presence of various anionic, cationic, and nonionic surfactants was also conducted to determine if they could improve analysis. However, the surfactants employed did not seem to have any meaningful enhancement; in fact, some actually diminished the fluorescence intensity of riboflavin. The procedure was linear for riboflavin from 0.01 to 2.5 &#956;g/mL. Reproducibility expressed as relative standard deviation was about 2%. The recoveries obtained range from 91.3% to 100.21% for the samples determined. The proposed method was successfully applied to the analysis of riboflavin in commercial vitamin tablets and cereal products. The results obtained were consistent with expected values as provided by the manufacturers. The method is simple, sensitive, economical, and rapid.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2008

Author dc:creator

• Ghann, William Emmanuel

• Surfactant
• Riboflavin
• Cereal
• Fluorescence
• Vitamin B2
• Fluorometer
• Analytical Chemistry
• Chemistry
• Physical Sciences and Mathematics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/2000

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3352