Toward the Synthesis of Nuclease Models.

<p>Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general acid-base activities are believed to be essential in multifunctional catalyses. Combination of such interactions in model compounds often resulted in larger than additive effects.</p><p>This work is aimed at synthesizing nuclease models that combine the ability to recognize phosphate groups and/or nitrogen bases of DNA together with the ability to catalyze phosphodiester hydrolysis. These models were designed to achieve optimum interaction between the recognition and the catalytic functionalities. Towards this goal, we chose phenonthiazonium ions (methylene blue analogues) and anthracene as spacers.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2008

Author dc:creator

• Fomumbod, Enni Nina

• synthetic probe
• metallonuclease
• Synthetic nuclease
• Chemicals and Drugs
• Enzymes and Coenzymes
• Medicine and Health Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1912

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3264