Differential Expression of Genes During Diapause in the Flesh Fly, <em>Sarcophaga crassipalpis</em>.

<p>The objective of this study was to identify genes that are differentially regulated during diapause when compared with nondiapausing pupae in <em>Sarcophaga crassipalpis</em>. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both subtractive libraries were isolated and the inserts sequenced. The sequences were analyzed using the bioinformatics tools NCBI, BlastX, Clustal W, etc. Out of 384 clones, 59 genes were found to be upregulated during diapause and 37 genes were found to be upregulated during a nondiapause pupal stage, no genes were found to be expressed commonly in both the diapause and nondiapause constructed libraries.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2009

Author dc:creator

• Karki, Puja

• Diapause
• Suppression Subtractive Hybridization
• Sarcophaga crassipalpis
• Genetics and Genomics
• Life Sciences
• Molecular Genetics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1818

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3170