Inhibition of <em>Escherichia coli</em> ATP Synthase by Polyphenols and Their Derivatives.

<p>We have studied the inhibitory effect of natural and structurally modified polyphenols on <em>Escherichia coli</em> ATP synthase to test (I) if the beneficial dietary effects of polyphenols are related to their inhibitory actions on ATP synthase, (II) if inhibitory effects of polyphenolic compound could be augmented through structural modifications, and (III) if they can act as antimicrobial agent through their actions on ATP synthesis. X-ray crystal structures of polyphenol binding sites suggested that polyphenols bind at a distinct polyphenol binding pocket, at the interface of &#945;,&#946;,&#947;-subunits. We found that both natural and modified polyphenols inhibit <em>E. coli</em> ATP synthase to varying degrees and structural modifications resulted in augmented inhibition. Inhibition was reversible in all cases. Both natural and modulated compounds inhibited <em>E. coli</em> cell growth to varying degrees. We conclude that dietary benefits of polyphenols may be in part due to the inhibition of ATP synthase.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2010

Author dc:creator

• Dadi, Prasanna Keerthi

• resveratrol
• polyphenols
• E coli
• F1Fo-ATP synthase
• F1-ATPase
• ATP synthesis
• quercetin
• biological nanomotor
• quercetin-3-β-D glucoside
• quercitrin
• piceatannol
• Bacteriology
• Life Sciences
• Microbiology

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1704

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3059