The Determination of Lead and Cadmium in Tobacco Using High Performance Liquid Chromatography and Dithiocarbamate Derivitization.

<p>A reversed phase-high performance liquid chromatography method has been developed that is capable of resolving lead and cadmium diethyldithiocarbamate complexes. The method does not require the presence of hazardous solvents to optimize peak shape and resolution; the mobile phase consists of methanol, water, and a surfactant. Tobacco samples were chemically oxidized and reacted with sodium diethyldithiocarbamate reagent prior to analysis using the HPLC method. The lead diethyldithiocarbamate determination was compromised by the presence of a significant interference in the analysis; lead determinations in 10 foreign and domestic tobacco samples ranged from 27.14 to 134.84 &#956;g/g. The cadmium diethyldithiocarbamate determination was not adversely affected by interferences. Cadmium determinations in 10 foreign and domestic tobacco samples ranged from 0.89 to 6.96 &#956;g/g. The tobacco samples were also analyzed using atomic absorption spectrometry. Foreign tobacco brands that contained clove as a spice showed lower levels of cadmium and lead.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2011

Author dc:creator

• Klein, Mark Stephen

• Diethyldithiocarbamate
• Heavy Metals
• High Performance Liquid Chromatorgraphy
• Tobacco
• Analytical Chemistry
• Chemistry
• Physical Sciences and Mathematics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1240

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2431