Expression, Cloning, and Sequencing of Putative Insulin Signaling Genes Involved in Diapause in the Flesh Fly <em>Sarcophaga crassipalpis</em>.

<p>Diapause is a programmed developmental arrest that allows insects to survive harsh environmental conditions. The diapause state has been linked to the insulin signaling pathway. Insulin signaling has been associated with many physiological processes including aging. It is a working hypothesis that the diapause and aging programs have a common set of gene expression pathways via insulin signaling. Analysis of a heterologous microarray indicated that two genes involved in the insulin pathway were down regulated during diapause. Both of these genes, Pi3K68d and Pde6, along with the Insulin receptor (InR) were targets for further investigation. Putative gene products have been isolated, cloned, and sequenced. RNA interference experimentation was conducted to characterize the role of the putative InR gene products obtained.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2005

Author dc:creator

• Barker, Andrew Bellamy

• Phosphodiesterase 6
• Phosohotidylinositol 3-kinase
• Insulin Signaling
• Diapause
• Biochemistry, Biophysics, and Structural Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1041

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2198