Characterization of a Catechol-Type Siderophore and the Detection of a Possible Outer Membrane Receptor Protein from <em>Rhizobium leguminosarum</em> strain IARI 312.

<p>Many gram-negative bacteria produce and secrete siderophores under iron-deficient conditions. Siderophores are low molecular weight compounds (600-1500 Daltons), which chelate ferric iron with an extremely high affinity, and the complex is actively transported across the outer and inner membranes of gram-negative bacteria. There are two main classes of siderophores: catechol and hydroxamate. Catechol-type siderophores chelate ferric iron via hydroxyl groups, and hydroxamate-type siderophores chelate ferric iron via a carbonyl group with an adjacent nitrogen. Rhizobia fix atmospheric nitrogen symbiotically in leguminous plants using the iron-containing enzyme nitrogenase. To satisfy their iron requirements, many rhizobia are known to produce siderophores. <em>Rhizobium leguminosarum</em> Strain IARI 312 is known to infect pigeon pea plants. <em>R. leguminosarum</em> Strain IARI 312 produces both a catechol-type and a hydroxamate-type siderophore when grown under iron deficient conditions. The catechol-type siderophore has been purified and chemically characterized, and is consistent with that of enterobactin.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2004

Author dc:creator

• Clark, Brianne Lee

• Rhizobium leguminosarum
• Siderophore
• Iron Acquisition
• Catechol
• Enterobactin
• Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/922

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2079