East Tennessee State University
Flavone: the Molecular and Mechanistic Study of How a Simple Flavonoid Protects DNA from Oxidative Damage.
Abstract
dc:description.abstract<p>Dietary flavonoids are ubiquitous and are marketed as supplements. Characterized as antioxidants, they offer protection against a number of degenerative diseases. Flavonoid mechanics involve free radical scavenging, metal chelation, and substrate association. The skeletal structure of flavonoids is a fused ring system modified by hydroxyl, sugar, and carbohydrate additions. Flavone is a structurally simple flavonoid. Quercetin and its glycosidic analog rutin are complex structures. Using a DNA oxidation/cleavage assay, flavone reduces DNA nicking by 91%. Depending on the solvent system used, quercetin can either increase or decrease DNA oxidation. Rutin exhibits neither pro- nor antioxidant activity. The molecular interactions responsible for these results are defined for flavone. 1) Flavone intercalates into DNA and saturates DNA at a 1/3.5 flavone:DNA molar ratio. 3) Flavone reduces iron-dependent DNA oxidation. 4) Flavone interacts with quercetin to enhance DNA protection. These results characterize the primary activities of a simple flavonoid.</p>
Degree
thesis:*- Name thesis:degree_name
- MS (Master of Science)
- Level thesis:degree_level
- Thesis - unrestricted
- Discipline thesis:degree_discipline
- Biomedical Sciences
- Year dc:date.issued
- 2003
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Dean, Jennifer Dawn
Subjects
dc:subject × 8Rights
dc:rights- Statement dc:rights
-
- Copyright by the authors.
Identifiers
dc:identifier.*- Repository record dc:identifier
- https://dc.etsu.edu/etd/849
- OAI identifier oai:identifier
- oai:dc.etsu.edu:etd-2006